Helena SPIFE 4000
Total Automation for IFEs and SPEs
Yes, you really can just load the sample tubes, press go, and return for your printed IFE and SPE results! SPIFE 4000 eliminates ALL manual steps for these assays – there’s no sample application, no stains or antisera to add, no gel transfers, no dilutions to perform, no gel blocks to remove, no tech-to-tech variability – just load and walk away. A full day’s mixed run of SPEs and IFEs takes about 30 minutes of tech time for set up, then frees the tech to perform other tasks. In a single 8-hour shift, SPIFE 4000 can turn 100 IFEs or 756 SPEs. With SPIFE 4000’s hands-off approach, a laboratory could reasonably expect to be able to run 24 hours per day 7 days a week
Auto Sampling for SPE & IFE -
• Liquid level sensing
• Auto dilutions for immunofixation
• Positive ID from tube to gel to final report

Auto Applicator and Electrophoresis -
• Auto sample application to gels
• Application system unaffected by triglycerides or large proteins
• Peltier-driven temperature controlled chamber

Integrated Auto Scanning -
• Automatic setting of scan parameters
• Full editing and interpretation capabilities
• Final results can be printed and/or exported to LIS

Auto IFE with SPIFE 4000
SPIFE 4000 automatically performs all the steps for immunofixation.
Auto Dilutions – the sample handler performs all the needed dilutions and applies samples to gels
Auto Antisera - Antisera are automatically applied to gels. Affinity and avidity of the antisera are balanced for best sensitivity with the least amount of blush.
Auto Press and Blot – Assures clear backgrounds, before moving into the auto stainer
Throughput – Four profiles per gel; up to 100 IFEs per 8 hours
Auto SPE with SPIFE 4000
No protein retention – application system is unaffected by triglycerides or large proteins.
Active cooling – excellent split-beta separation with no degradation
Great results – resolution and presentation are excellent
Throughput – 28 samples per gel; up to 756 per 8 hours
SPIFE 4000 Hemoglobins
Alkaline Hb

SPIFE 4000 can perform alkaline hemoglobin separation for eight samples per gel, with three gels per run.

• Aids in identifying hemoglobinopathies
• Stable in storage with acid blue stain
• Automated from sample application to finished gel
Acid Hb
Separate up to 14 acid hemoglobin samples per gel and up to three gels per run on SPIFE 4000.

• Aids in identifying hemoglobinopathies
• Stable in storage with acid blue stain
• Excellent separations
Hb IEF
SPIFE 4000 automates isolectric focusing of hemoglobins on up to 14 samples from sample application to finished gel.

• Produces crisp, sharply resolved hemoglobin bands
• Aids in distinguishing hemoglobin variants
• Final results in 75 minutes